Biology Practical: Osmosis & Diffusion

 




TRANSPORTATION OF MATERIALS IN LIVING THINGS

Osmosis

This is the movement of water molecules from a region of higher concentration to a region of lower concentration through a semi- permeable membrane.

 

A partially-permeable membrane is a membrane that allows small particles such as water molecules to pass through it, but not larger particles such as sugar molecules and ions from salts. Examples of semi-permeable membranes are cell membranes and a pig’s bladder.

 

For osmosis to take place there must be:

1.      Two solutions with different concentrations; and

2.      A partially permeable membrane to separate them.

 

Effects of osmosis in animal cell

Red blood cells in Isotonic Solution

Ø  If the red blood cell placed in isotonic solution, the cell will neither gain nor lose water

Ø  This means that the movement of water into and out the cell by osmosis will be the same

 

Red blood cell in hypotonic solution

Ø  If the red blood cell placed in hypotonic solution, the cell will gain water by osmosis

Ø  This will cause the cell to swell and eventually burst due building up of osmotic pressure in cytoplasm

Ø  Under extreme condition the red blood cell become haemolysed


Haemolysis is the process by which red blood cell burst when placed in hypotonic solution

 

Red blood cell in hypertonic solution

Ø  If the red blood cell placed in hypotonic solution, the cell will lose water by osmosis

Ø  This will cause the cell to shrink and became crenated

Crenation is process by which red blood cell loses water and shrink when placed in hypertonic solution



 



Effect of osmosis in plant cells

Plant cell in isotonic solution

Ø  If the plant cell placed in isotonic solution, the cell will neither gain nor loses water

Ø  This means that the movement of water into and out the cell by osmosis will be the same


Plant cell in hypotonic solution

Ø  If the plant cell placed in hypotonic solution, the cell gain water by osmosis

Ø  The cell does not burst due to the presence of rigid cell wall

Ø   The cell become firm and rigid and is said to be turgid. Turgidity is the condition where by a plant cell is full of water such that it can no longer absorb any more water from surrounding

Plant cell in hypertonic solution

Ø  If the plant cell placed in hypotonic solution, the cell will lose water by osmosis

Ø  This will cause the cell to shrink and became flaccid

Ø  This cause the plant to became floppy and wilt due to lack an internal support

Ø  Under extreme case the plant became plasmolysed

Plasmolysis is the process by which plant cell loses water, shrink and becomes flaccid when placed in hypertonic solution





Osmosis in unicellular organisms

Unicellular organisms that live in fresh water, for example amoeba and euglena, are hypertonic to their surrounding so water enters the organisms by osmosis. These organisms have a contractile vacuole.

 

The contractile vacuole collects the excess water and removes it from the cell. This prevents the cell from bursting

 

Sample practical question

Aim of the experiment: To demonstrate the process of osmosis using potato cubes

Materials: Three raw potatoes, sugar solution, water and beakers or dishes

Procedures

1.    Boil one of the potatoes (boiling kills the cells).

2.    Cut each potato in half and make a shallow hole in each half

3.    Place each potato half in a beaker or dish that has water. The water should not cover the potato.

4.    Put some sugar solution in the shallow hole in the one raw potato and the boiled potato. Leave the other potato empty. Label the set-ups (a),(b) and (c)

5.    Mark the level of water in each beaker or dishes

6.    Leave the setups undisturbed overnight

 

Observation



 

Diffusion

Diffusion is the net movement of particles from the region of high concentration to low concentration until the particles are evenly distributed.

Factors affecting the rate of Diffusion

i.         Concentration gradient; A huge difference between the concentration of the molecules in two regions leads to a faster rate of diffusion

ii.       Surface area to volume ration; the higher the ratio the greater the rate of diffusion

iii.     Temperature; increase in temperature increases the rate of diffusion and decrease in temperature decreases the rate of diffusion

iv.     Thickness of the membrane; thin membrane enhances the higher rate of diffusion than the thick membrane

v.       Size of molecules; small and light molecules diffuses faster than large and heavy molecules


The role of diffusion in living things

i.         Absorption; digested food example amino acids and glucose diffuse across the walls of the ileum to the blood system. Also plants absorb most of mineral salts by diffusion

ii.       Gaseous exchange; the process of gaseous exchange in plants and animals occurs through diffusion

iii.     Excretion of waste; waste products move out of the cell through diffusion

Sample practical question

Experiment: Demonstrating Diffusion using Potassium Permanganate Crystals

Materials:

i.       Glass beaker or clear glass container

ii.       Potassium permanganate crystals

iii.       Water

iv.       Stirring rod or glass rod

v.      Stopwatch or timer

vi.       Paper or cardboard

 

Procedure:

1.       Fill the glass beaker or container with water, about two-thirds full.

2.       Add a few potassium permanganate crystals to the water. The crystals will slowly dissolve, turning the water purple.

3.       Use the stirring rod to gently stir the water and potassium permanganate mixture.

4.       After stirring, observe the initial distribution of the purple color in the water.

 

Questions to Explore:

1.    What happens when potassium permanganate crystals are added to water?

2.    Why does the water turn purple when potassium permanganate is added?

3.    Describe the initial distribution of the purple color in the water after stirring.

4.    What do you think will happen to the purple color in the water over time? Why?


5.    How does the purple color spread throughout the water? What process is responsible for this movement?

6.    What do you notice about the movement of the purple color particles in the water? Is it uniform, or does it show any specific pattern?

7.    How can you explain the process responsible for the spreading of the purple color in the water?

8.    Does the movement of the purple color particles depend on the presence of an external force, like stirring? Why or why not?

9.    What factors do you think can affect the rate of color spreading in the water? How can you design an experiment to test this?

10.   Can you compare the diffusion process observed here to any natural processes you know of? How are they similar or different?

 

Differences between diffusion and osmosis

DIFFUSION

OSMOSIS

Diffusion is the movement of materials from the area of their higher concentration to the area of their lower concentration

Osmosis is the movement of only solvents or water from the area of their lower concentration to the area of their higher concentration through a semi permeable

membrane

Diffusion can operate in any

medium

Osmosis operates only in

liquid medium

Diffusion is applicable to all types substances (solids

liquids and gases)

Osmosis is applicable only to the solvent part of the

solution

Diffusion does not require a

semi permeable membrane

Osmosis requires the semi

permeable membrane

It helps to equalize the concentration of the diffusing substances throughout the available

space

It does not equalize the concentration of the substance on two sides of the system